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 Mouse cloned from drop of blood

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PostSubject: Mouse cloned from drop of blood   Fri 28 Jun 2013, 20:32

Mouse cloned from drop of blood

By Helen Briggs BBC News
The mouse was cloned from a blood cell
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Scientists in Japan have cloned a mouse from a single drop of blood.
Circulating blood cells collected from the tail of a donor mouse were used to produce the clone, a team at the Riken BioResource Center reports in the journal Biology of Reproduction.
The female mouse lived a normal lifespan and could give birth to young, say the researchers.
Scientists at a linked institute recently created nearly 600 exact genetic copies of one mouse.
Mice have been cloned from several different sources of donor cells, including white blood cells found in the lymph nodes, bone marrow and liver.
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This technique would be applicable for generating genetic copies of invaluable strains of mice”
Research team Riken BioResource Center, Tsukuba
The Japanese research group investigated whether circulating blood cells could also be used for cloning.
Their aim was to find an easily available source of donor cells to clone scientifically valuable strains of laboratory mice.
The team, led by Atsuo Ogura, of Riken BioResource Center in Tsukuba, took blood from the tail of a donor mouse, isolated the white blood cells, and used the nuclei for cloning experiments, using the same technique that produced Dolly the sheep in Edinburgh.
The process, known as somatic cell nuclear transfer, involves transferring the nucleus from an adult body cell - such as a blood or skin cell - into an unfertilised egg that has had its nucleus removed.
Reporting their findings in the US journal, Biology of Reproduction, the scientists said the study "demonstrated for the first time that mice could be cloned using the nuclei of peripheral blood cells".
'Invaluable strains'
They added: "These cells could be used for cloning immediately after collection and no donor animals need to be euthanised.
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The cloning method - somatic cell nuclear transfer

  • Clones of adult animals are produced by a method called somatic cell nuclear transfer, which refers to the transfer of the nucleus from a somatic cell into an egg cell
  • A somatic cell is any cell of the body apart from a germ (sex) cell
  • In cloning, the nucleus of a somatic cell is removed and inserted into a donor unfertilised egg that has had its own nucleus (containing the genetic material) removed
  • The embryo is then placed inside a surrogate mother.

"This technique would be applicable for generating genetic copies of invaluable strains of mice, which cannot be preserved by other assisted reproductive techniques such as conventional in vitro fertilisation or intracytoplasmic sperm injection."
Scientists in Japan have years of experience in cloning mice.
A team at a linked institute recently revealed they had produced almost 600 mice from one donor mouse after 25 consecutive rounds of cloning.
The research is aimed at large-scale production of high-quality animals for farming or conservation purposes, they say.
Commenting on the study, Professor Robin Lovell-Badge of the MRC National Institute of Medical Research in London, said it was a useful small advance on previous work showing that it is possible to clone mice from a variety of adult cell types, including white blood cells.
He told BBC News: "The efficiency of cloning from these cell types was very good, suggesting that even a small drop of blood will contain sufficient numbers...this is helpful if the intention is to use cloning to propagate and expand numbers of rare or valuable types of individual or species."

source:-
http://www.bbc.co.uk/news/health-23068423
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